Application

lipid and glucose metabolism, exercise, insulin action, cell signaling

Cell Line Origin

Muscle (Limb)

Components

Basal Medium containing 10% FBS & 10% DMSO

General description

Human Skeletal Muscle Cells (HSkMC) are isolated from the skeletal muscle of limbs from adult or fetal donors. Human Skeletal Muscle Cells can undergo differentiation to exhibit actin and myosin myofilaments. HSkMC provide a useful model system to study many aspects of muscular function and disease. Skeletal Muscle Cells play an instrumental role in the glucose metabolism and diabetes, therefore select HSkMC lots undergo an additional set of characterization to demonstrate AMPK signaling and responsiveness to insulin stimulation.

Preparation Note

3rd passage, >500,000 cells in Basal Medium containing 10% FBS & 10% DMSO Can be cultured at least 15 doublings

Subculture Routine

Please refer to the HSkMC Culture Protocol.